Here are the details of how we will conduct our experiment and the measurements we will make: Our group will calculate the percentage of seeds germinated in each trial by using a basic formula. We will count the total number of seeds for each concentration, count the number of seeds that germinate and use these numbers to figure out the percentage using a calculator.
In this experiment, cress seeds are to be used. Going through the basics first though, for a seed to successfully germinate there needs to be: Water absorption is imbibition. The optimum temperature for germination is the optimum for the enzymes involved in mobilisation of food reserves, provided that other factors are not limiting.
This temperature varies from species to species i. Respiration makes available the energy for metabolism and growth. Germinating seeds respire very rapidly, and require oxygen for aerobic respiration. Seeds will not germinate in the total absence of oxygen. Diffusion of oxygen through the testa may be slow, however, and in the early stages of germination seeds may rely on some anaerobic respiration, at least until the testa has ruptured.
So this explains why water, oxygen and a suitable temperature are needed for germination, therefore needed for this experiment. Light is obviously the last condition that Sorry, but full essay samples are available only for registered users comes into place though not in all seeds with the cress seeds being used in this experiment.
Inhibitors A germination inhibitor is to be used in this experiment. The hypothesis is that it will affect the germination of the seed. Inhibitors are not just used in plants; they are also used in enzymes Enzyme inhibitors.
They alter the rate of enzyme catalysed reactions. The reactions of enzymes may be enhanced or inhibited by various substances, some formed in the cell and others absorbed from the external environment. This is similar to the water-soluble protein germination inhibitor found in tomatoes because this will also alter the rate of reaction; it will alter the rate of germination in the seeds.
Enzymes can be denatured when the temperature rises above their specific optimum temperature because the molecules vibrate so much that the bonds break and so the structure and shape is changed. So this germination inhibitor will have an optimum temperature and, like enzymes, will stop working to its particular function when this temperature is exceeded.
This then enables the seed to release the germination promoting plant hormone, gibberellic acid. This acts on the aleurone layer inside the seed coat to stimulate alpha-amylase production.
This enzyme then starts to break down the stored starch water insoluble in the endosperm of the seed embryo into simpler water soluble sugars.
The embryo meristem tissues use the energy from these sugars to begin cell division and the seed then sprouts. Some inhibitors induce dormancy in the embryo therefore not allowing this to happen this is mentioned below. Stored food and the mobilisation of Seeds need particular substances inside them for them to germinate.
The stored food of seeds consists of carbohydrates, lipids and proteins. Food is stored in seeds in an insoluble form, and must be hydrolysed to soluble substances early in germination, following the hydration of the seed.
Stored food is hydrolysed to produce the substrates for respiration sugars and the building blocks for synthesis substances such as sugars, amino acids and fatty acids. Some inhibitors alter germination in seeds by inducing dormancy in the embryo. The Experiment To carry out this experiment, all instructions must be carried out carefully and precise measurements given should be used.
That in high concentration conditions of inhibitor then the cress seeds will germinate very slowly or none at all — showing a relationship between inhibitor concentration and germination.
That there is no relationship between the concentration of the inhibitor and the germination of cress seeds.
The Variables The independent variable is the one that is going to be changed, which is the Inhibitor Concentration. This is being changed by mixing it in with varying amounts of water to dilute the solution so it becomes weaker and therefore the concentration is varied.
The dependant variable is the one that is to be measured as a result, which is the cress seed height.
Height is being used to determine the germination rate because it can be easily and accurately measured using a ruler and provides clear results that can be analysed. The controlled variables are all the other factors which could affect the results.
These are to be kept constant:An Investigation Into the Inhibiting Effect of Tomato Juice on the Germination of Cress Seeds Words | 20 Pages.
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The cress seeds in my experiment that are in a solution that lacks Mg have the darkest foliages. This could be caused because the workss found that the Sulphur in the solution counter acted the effects of the deficiency of Mg.